KDEL Receptor Antibody (OASE00040)

Data Sheet
 
Product Number OASE00040
Product Page www.avivasysbio.com/kdel-receptor-antibody-oase00040.html
Name KDEL Receptor Antibody (OASE00040)
Clone KR-10
Isotype IgG1
Conjugation Unconjugated
NCBI Gene Id 618184
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Gene Full Name KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
Description The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
Alias Symbols ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23
Reference 1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737.
2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219.
3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316.
4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328.
5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163.
6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
Reconstitution and Storage Store at -20C. Shipping with Blue Ice or 4C.
Datasheets/Manuals Printable datasheet for OASE00040
Specificity Detects ~25kDa.
Additional Information Background Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.
Additional Information Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4).
In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
Immunogen A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
Additional Information Certificate of Analysis: 1ug/ml was sufficient for detection of KDEL receptor in 20ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Dilution WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Uniprot ID P33946
Protein Accession # NP_001069963.1
Purification Protein G Purified
Gene Symbol KDEL Receptor
Predicted Species Reactivity Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Pig, Sheep, Dog, Chicken, Drosophila, Xenopus
Application WB, IHC, ICC, IF, IP
Image 1
Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.
Image 2
Western Blot
Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
 

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