Goat Anti-Human IgA Antibody - HRP Conjugated (OASB01837)

Data Sheet
 
Product Number OASB01837
Product Page www.avivasysbio.com/goat-anti-human-iga-antibody-hrp-oasb01837.html
Name Goat Anti-Human IgA Antibody - HRP Conjugated (OASB01837)
Isotype IgG
Conjugation HRP
Host Goat
Clonality Polyclonal
Concentration Lot specific
Gene Full Name B-cell antigen receptor complex-associated protein alpha chain
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Other Applications Data Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for Goat Anti-Human IgA Antibody - HRP Conjugated (OASB01837)
Specificity IgA
Additional Information Description: Pooled antisera from goats hyperimmunized with human IgA; purified by affinity chromatography on human IgA covalently linked to agarose; reacts with the heavy chain of human IgA; cross-adsorbed against human IgG and IgM for minimal reactivity
Application Info Direct immunofluorescent staining of IgA+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting
Immunogen Human IgA
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Immunofluorescence:
FLISA:
ELISA:
FITC conjugate
BIOT conjugate
R-PE conjugate
TRITC and TXRD conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:100 – 1:400
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Cross Absorption Human IgM and IgG.
Characterization To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent.
Purification Affinity chromatography on pooled human IgA covalently linked to agarose.
Tested Species Reactivity Human
Predicted Species Reactivity Human
Application ELISPOT, IHC-F, IHC-P, ICC, WB
Image 1
Purified human IgA, IgG, and IgM
ELISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-HRP.
 

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