Mouse CD154/CD40L Antibody (OASB01689)

• Product Number: OASB01689
• Name: Mouse CD154/CD40L Antibody (OASB01689)
• Clone: MR1
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 21947
• Host: Hamster
• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Gene Full Name: CD40 ligand
• Alias Symbols: IGM, IMD, gp3, Cd40, HIGM, IMD3, Ly-6, Ly62, T-BA, TRAP, Tnfs, gp39, CD154, Cd40l, HIGM1, Ly-62, T-BAM, CD40-L, Tnfsf5, Tnlg8b
• Reference: 1. Noelle, R.J., M. Roy, D.M. Shepherd, I Stamenkovic, J.A. Ledbetter, and A. Aruffo. 1992. Proc. Natl. Acad. Sci. U.S.A. 89:6550
  2. Aruffo, A., M. Farrington, D. Hollenbaugh, X. Li, A. Milatovich, S. Nonoyama, J. Bajorath, L.S. Grosmaire, R. Stenkamp, and M. Neubauer
  1993. Cell 72:291
  3. Hollenbaugh, D., L.S. Grosmaire, C.D. Kullas, N.J. Chalupny, S. Braesch- Andersen, R.J. Noelle, I. Stamenkovic, J.A. Ledbetter, and A. Aruffo
  1992. EMBO J. 11:4313
  4. Foy, TM., DM Shepherd, F.H. Durie, A. Aruffo, J.A. Ledbeter, and R.J. Noelle. 1993. J. Exp. Med. 178:1567
  5. Foy, T.M., D.M. page, T.J. Waldschmidt, A. Schoneveld, J.D. Laman, S.R. Masters, L. Tygrett, J.A. Ledbetter, A. Aruffo, and E. Claassen
  1995. J. Exp. Med. 182:1377
  6. Roy, M., T.J. Waldschmidt, A. Aruffo, J.A. Ledbetter, and R.J. Noelle. 1993. J. Immunol. 151:2497
  7. Armitage, R.J., W.C. Fanslow, L. Stockbine, T.A. Sato, K.N. Clifford, B.M. Macduff, D.M. Anderson, S.D. Gimpel, T. Davis- Smith, and C.R
  Maliszewski. 1992. Nature 357:80.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Other Applications Data: Since applications vary, you should determine the optimum working dilutionfor the product that is appropriate for your specific need.
• Specificity: CD154
• Additional Information: Description: CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. It is an important accessory molecule in T cell-B cell costimulatory interactions and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporin-sensitive. The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens yet does not alter the immune response to TI antigens.
• Application Info: Flow cytometry 1, 5, Immunoprecipitation 1, In vivo and in vitro blockage of CD154 function 1, 3, 5
• Other Applications Image 1 Data: Amount Used: 2 Ug/106 cells Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or platebound hamster anti-mouse CD3epsilon for 7 hours at 37 C. The cells were then harvested, double-stained with anti-CD154/CD
• Immunogen: Activated mouse Th1 clone D1.6
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate <= 2 ug/10⁶ cells
  Biotin conjugate <= 2 ug/10⁶ cells
  R-phycoerythrin conjugate <= 0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
• Uniprot ID: P27548
• Protein Name: CD40 ligand
• Protein Accession #: NP_035746.2?
• Nucleotide Accession #: NM_011616.2
• Tested Species Reactivity: Mouse
• Gene Symbol: CD40LG
• Predicted Species Reactivity: Mouse
• Application: FC, IHC-F, IP, BL
• Image 1: s spleen T BALB/c mice
  
  Amount Used: 2 Ug/106 cells Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or platebound hamster anti-mouse CD3ε for 7 hours at 37 C. The cells were then harvested, double-stained with anti-CD154/CD40L-FITC and anti-CD4+8-R-PE, and analyzed by flow cytometry,