Goat Anti-Mouse IgG3 Antibody (OASB01625)

• Product Number: OASB01625
• Name: Goat Anti-Mouse IgG3 Antibody (OASB01625)
• Isotype: IgG
• NCBI Gene Id: 380795
• Host: Goat
• Clonality: Polyclonal
• Concentration: 1.0 mg/mL
• Gene Full Name: Ig gamma-3 chain C region
• Alias Symbols: IgG, IgG3, AI3240
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) conjugate is supplied as 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - R- phycoerythrin- Cyanine 7 (R- PE- CY 7) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates aresupplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
• Other Applications Data: Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
• Specificity: IgG3
• Additional Information: Description: Pooled antisera from goats hyperimmunized with mouse IgG3; purified by affinity chromatography on mouse IgG3 covalently linked to agarose; reacts with the heavy chain of mouse IgG3; cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity
• Application Info: Indirect immunostaining in conjunction with mouse IgG3 monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting
• Other Applications Image 1 Data: Amount Used: u1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After
• Immunogen: Mouse IgG3
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Immunofluorescence:
  ELISA:
  FITC, TRITC, TXRD BIOT, and CY5 conjugates
  R-PE, SPRD, R-PE- CY7, and APC-CY7 conjugates
  AP conjugate
  HRP conjugate
  BIOT conjugate
  <= 1 ug/10⁶ cells
  <= 0.1 ug/10⁶ cells
  1:2,000-1:4,000
  1:4,000-1:8,000
  1:5,000-1:20,000
• Cross Absorption: Mouse IgM, IgG1, IgG2a, IgG2b and IgA, pooled human sera and purified humanparaproteins.
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
• Uniprot ID: P03987
• Purification: Affinity chromatography on mouse IgG3 covalently linked to agarose.
• Tested Species Reactivity: Mouse
• Gene Symbol: IGHG3
• Application: FC, IHC-F, IHC-P, ICC, WB, ELISPOT
• Image 1: Purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA and human IgG, IgM, and IgA
  
  ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG3, Human ads-UNLB followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP.
• Image 2: Human peripheral blood mononuclear
  
  Amount Used: u1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse IgG3-FITC, following which lymphocytes were gated and analyzed by flow cytometry.