Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)

Data Sheet
 
Product Number OASB01607
Product Page www.avivasysbio.com/goat-anti-mouse-igg2b-human-ads-antibody-ap-oasb01607.html
Name Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)
Isotype IgG
Conjugation AP
NCBI Gene Id 16016
Host Goat
Clonality Polyclonal
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Gene Full Name Ig gamma-2B chain C region
Alias Symbols IgG2, IgG2b, Igh-3, gamma2b
Product Format Liquid 50mM Tris/1mM MgCl2 and 50% glycerol (pH 8.0) containing sodium azide as preservative
Reconstitution and Storage Store at 2-8°C or long-term at -20°C.
Other Applications Data Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)
Specificity Reacts with the heavy chain of mouse IgG2b. Mouse IgG1, IgG2a, IgG3, IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species.
Application Info ELISA: 1:2000~4000
Other Applications Image 1 Data Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After
Immunogen Mouse IgG2b
Warning Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Cross Absorption Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins.
Characterization To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Uniprot ID P01867
Purification Affinity chromatography on mouse IgG2b covalently linked to agarose
Tested Species Reactivity Mouse
Gene Symbol IGHG2B
Predicted Species Reactivity Mouse
Application IHC-F, IHC-P, ICC, WB, ELISPOT
Image 1
Human peripheral blood mononuclear
Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse IgG2b-FITC, following which lymphocytes were gated and analyzed by flow cytometry.
Image 2
Purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA
ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b, Human ads-AP.
 

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