Product Number |
OASB01607 |
Product Page |
www.avivasysbio.com/goat-anti-mouse-igg2b-human-ads-antibody-ap-oasb01607.html |
Name |
Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607) |
Isotype |
IgG |
Conjugation |
AP |
NCBI Gene Id |
16016 |
Host |
Goat |
Clonality |
Polyclonal |
Source |
Pooled antisera from goats hyperimmunized with mouse IgG2b |
Gene Full Name |
Ig gamma-2B chain C region |
Alias Symbols |
IgG2, IgG2b, Igh-3, gamma2b |
Product Format |
Liquid 50mM Tris/1mM MgCl2 and 50% glycerol (pH 8.0) containing sodium azide as preservative |
Reconstitution and Storage |
Store at 2-8°C or long-term at -20°C. |
Other Applications Data |
Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
Datasheets/Manuals |
Printable datasheet for Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607) |
Specificity |
Reacts with the heavy chain of mouse IgG2b. Mouse IgG1, IgG2a, IgG3, IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species. |
Application Info |
ELISA: 1:2000~4000 |
Other Applications Image 1 Data |
Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After |
Immunogen |
Mouse IgG2b |
Warning |
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Cross Absorption |
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
Characterization |
To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. |
Uniprot ID |
P01867 |
Purification |
Affinity chromatography on mouse IgG2b covalently linked to agarose |
Tested Species Reactivity |
Mouse |
Gene Symbol |
IGHG2B |
Predicted Species Reactivity |
Mouse |
Application |
IHC-F, IHC-P, ICC, WB, ELISPOT |
Image 1 | Human peripheral blood mononuclear
| Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse IgG2b-FITC, following which lymphocytes were gated and analyzed by flow cytometry. |
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Image 2 | Purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA
| ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b, Human ads-AP. |
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