Goat Anti-Mouse IgA Antibody - TRITC Conjugated (OASB01537)

Data Sheet
 
Product Number OASB01537
Product Page www.avivasysbio.com/goat-anti-mouse-iga-antibody-tritc-oasb01537.html
Name Goat Anti-Mouse IgA Antibody - TRITC Conjugated (OASB01537)
Isotype IgG
Conjugation TRITC: Tetramethylrhodamine
NCBI Gene Id 238447
Host Goat
Clonality Polyclonal
Concentration 1.0 mg/mL
Gene Full Name B-cell antigen receptor complex-associated protein alpha chain
Alias Symbols I, IgA, Igh-, Igh-2
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 0.5 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Other Applications Data Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for anti-CD79A (OASB01537) antibody
Specificity IgA
Additional Information Description: Pooled antisera from goats hyperimmunized with mouse IgA; purified by affinity chromatography on mouse IgA covalently linked to agarose; reacts with the heavy chain of mouse IgA; cross-adsorbed against mouse IgG and IgM for minimal reactivity
Application Info Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry, Flow cytometry
Immunogen Mouse IgA
Warning Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution ELISA:
AP conjugate
HRP conjugate
BIOT conjugate
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
FLISA: TRITC and TXRD conjugates 1:100 – 1:400
Immunofluorescence:
FITC conjugate
R-PE conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
Cross Absorption Mouse IgG1, IgG2a, IgG2b, IgG3 and IgM.
Characterization To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
Uniprot ID A0A0A6YXW6
Purification Affinity chromatography on mouse IgA covalently linked to agarose.
Tested Species Reactivity Mouse
Gene Symbol IGHA
Application FLISA, IHC-F, IHC-P, ICC
Image 1
Purified mouse IgA, IgG, and IgM
FLISA plate was coated with purified mouse IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-TRITC.
Image 2
Paraffin embedded BALB/c mouse colon tissue section
Paraffin embedded BALB/c mouse colon tissue section was stained with Goat Anti-Mouse IgA-TRITC followed by DAPI.
 

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