Mouse Qa-2 Antibody (OASB00702)

• Product Number: OASB00702
• Name: Mouse Qa-2 Antibody (OASB00702)
• Clone: 695H1-9-9
• Isotype: IgG2a
• Conjugation: Unconjugated
• NCBI Gene Id: 15018
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Gene Full Name: H-2 class I histocompatibility antigen, Q7 alpha chain
• Alias Symbols: Pe, Q9, Qa, Ped, Qa-, Qa7, Qa-2, Qa-7, H-2Q7
• Reference: 1. Sharrow, S.O., J.S. Arn, I. Stroynowski, L. Hood, and D.H. Sachs. 1989. J. Immunol. 142:3495- 3502
  2. Sharabi, Y., and D.H. Sachs. 1990. J. Exp. Med. 171:211- 219.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed.
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Specificity: Qa-2
• Additional Information: Description: The 695H1-9-9 antibody reacts with a Qa-2-determined alloantigen. It recognizes an epitope distinct from that recognized by monoclonal antibody 1-1-2.
• Application Info: Flow cytometry 2, Complement-mediated cytotoxicity 2, Modulation of Qa-2 expression from peripheral T lymphocytes 2
• Immunogen: C3H.SW mouse skin graft and splenocytes
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Purified antibody
  Fluorescein conjugate
  Biotin conjugate
  R-phycoerythrin conjugate
  <=1 ug/10⁶ cells
  <=1 ug/10⁶ cells
  <=1 ug/10⁶ cells
  <=0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent.
• Uniprot ID: P14429
• Protein Name: H-2 class I histocompatibility antigen, Q7 alpha chain
• Protein Accession #: NP_034524.3?
• Nucleotide Accession #: NM_010394.4
• Tested Species Reactivity: Mouse
• Gene Symbol: H2-Q7
• Predicted Species Reactivity: Mouse
• Application: FC, ICC, CMCD
• Image 1: C57BL/6 mouse splenocytes
  
  C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse Qa-2-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-PE.