Mouse CD122 Antibody : Biotin (OASB00698)

• Product Number: OASB00698
• Name: Mouse CD122 Antibody : Biotin (OASB00698)
• Clone: 5H4
• Isotype: IgG2a
• Conjugation: Biotin
• NCBI Gene Id: 16185
• Host: Rat
• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Gene Full Name: Interleukin-2 receptor subunit beta
• Alias Symbols: p70, CD122, IL15R, Il-2R, IL-15R, IL15Rbeta, Il-2Rbeta, IL-15Rbeta, Il-2/15Rbeta
• Reference: 1. Malek, T.R., et al. 1995. J. Interferon Cytokine Res. 15:447
  2. Minami, Y., et al. 1993. Annu. Rev. Immunol. 11:245
  3. Taniguchi, T., and Y. Minami. 1993. Cell 73:5
  4. Tanaka, T., et al. 1991. J. Immunol. 147:2222.
• Description of Target: The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mLof PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Specificity: CD122
• Additional Information: Description: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (iii) the common gamma chain (gamma c) that does not bind IL-2. The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expressed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding.
• Application Info: Flow cytometry 1, Immunoprecipitation 1
• Other Applications Image 1 Data: Amount Used: 1 Ug/106 cells BALB/c lymph node cells were double stained with rat anti-mouse CD3e-R-PE and rat anti-mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
• Immunogen: Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-m-beta-t-28)
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate <= 1 ug/10⁶ cells
  Biotin conjugate <= 1 ug/10⁶ cells
  R-phycoerythrin conjugate <= 0.2 ug/10⁶ cells
  Allophycocyanin conjugate <= 0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative flow data are included in this productinsert.
• Uniprot ID: P16297
• Protein Name: interleukin-2 receptor subunit beta
• Protein Accession #: NP_032394.1?
• Nucleotide Accession #: NM_008368.4
• Tested Species Reactivity: Mouse
• Gene Symbol: IL2RB
• Predicted Species Reactivity: Mouse
• Application: FC, IP
• Image 1: BALB/c lymph node
  
  Amount Used: 1 Ug/106 cells BALB/c lymph node cells were double stained with rat anti-mouse CD3e-R-PE and rat anti-mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).