Mouse Ly-6C Antibody (OASB00560)

Data Sheet
 
Product Number OASB00560
Product Page www.avivasysbio.com/rat-anti-mouse-ly-6c-antibody-oasb00560.html
Name Mouse Ly-6C Antibody (OASB00560)
Clone HK1.4
Isotype IgG2c
Conjugation Unconjugated
NCBI Gene Id 17067
Host Rat
Clonality Monoclonal
Concentration 0.5 mg/mL
Gene Full Name Lymphocyte antigen 6C1
Alias Symbols Ly6, Ly-6, Ly6c, Ly-6C, Ly-6C1, AA682074, AA959465
Reference 1. Havran, W.L., D.W. Lancki, R.L. Moldwin, D.P. Dialynas, and F.W. Fitch. 1988. J. Immunol 140:1034
2. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, S. Fleming, L.A. Herzenberg, E.L. Berg, and E.C. Butcher. 1988. Eur. J. Immunol. 18:1819
3. Johnson, R., D.W. Lancki, and F.W. Fitch. 1993. J. Immunol. 151:2986
4. Lancki, D.W., P. Fields, D. Qian, and F.W. Fitch. 1995. Immunol. Rev. 146:117.
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed.
Other Applications Data Since applications vary, each investigator must determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Datasheets/Manuals Printable datasheet for Mouse Ly-6C Antibody (OASB00560)
Specificity Ly-6C
Additional Information Description: Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.
Application Info Flow cytometry 1, In vitro activation of T lymphocytes 1
Other Applications Image 1 Data Amount Used: 1 Ug/106 cells Bone marrow cells from C57BL/6 mice were labeled with purified rat anti-mouse Ly-6C and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.
Immunogen L3 cloned CTL cells
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry: Purified antibody
Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
<=1 ug/106 cells
<=1 ug/106 cells
<=1 ug/106 cells
<=0.2 ug/106 cells
Characterization To insure lot- to- lot consistency, each batch of product is tested by flow cytometry for conformance withcharacteristics of a standard reference reagent. Representative flow data are included in this product insert.
Uniprot ID P0CW02
Protein Name lymphocyte antigen 6C1
Protein Accession # NP_001238984.1?
Nucleotide Accession # NM_001252055.1
Tested Species Reactivity Mouse
Gene Symbol LY6C1
Predicted Species Reactivity Mouse
Application FC, IHC-F
Image 1
Bone marrowfrom C57BL/6 mice
Amount Used: 1 Ug/106 cells Bone marrow cells from C57BL/6 mice were labeled with purified rat anti-mouse Ly-6C and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.
 

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