Mouse CD80/B7-1 Antibody : FITC (OASB00518)

• Product Number: OASB00518
• Name: Mouse CD80/B7-1 Antibody : FITC (OASB00518)
• Clone: 1G10
• Isotype: IgG2a
• Conjugation: FITC
• NCBI Gene Id: 12519
• Host: Rat
• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Gene Full Name: T-lymphocyte activation antigen CD80
• Alias Symbols: B71, Ly5, Cd28, Ly-5, Ly53, TSA1, Cd28l, Ly-53, MIC17
• Reference: 1. Nabavi, N., G. J. Freeman, A. Gault, D. Godfrey, L. M. Nadler, and L. H. Glimcher. 1992. Nature 360:2662. Chen, C., D. A. Faherty, A. Gault, S. E. Connaughton, G. D. Powers, D. I. Godfrey, and N. Nabavi. 1994. J. Immunol. 152:2105
  3. Natesan, M., Z. Razi- Wolf, and H. Reiser. 1996. J. Immunol. 156:2783
  4. Razi- Wolf, Z., G. J. Freeman, F. Galvin, B. Benacerraf, L. Nadler, and H. Reiser. 1992. Proc. Natl. Acad. Sci. U. S. A. 89:4210
  5. Hathcock, K. S., G. Laszlo, C. Pucillo, P. Linsley, and R. J. Hodes. 1994. J. Exp. Med. 180:631
  6. Bluestone, J.A. 1995. Immunity 2:555
  7. Powers, G. D., D. A. Faherty, S. E. Connaughton, D. A. Biondi, D. I. Godfrey, A. Gault, C. Y. Chen, and N. Nabavi. 1994. Cell Immunol
  153:298.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Other Applications Data: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for yourspecific need.
• Specificity: CD80
• Additional Information: Description: CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor crosslinking with peak expression occurring between 48 and 72 hours post-activation. The interaction of CD80 with its ligands, CD28 and CD152 (CTLA-4), provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80 but it does not block stimulation of T cells by natural antigen-presenting cells.
• Application Info: Flow cytometry 2, 7, Immunoprecipitation 2, In vivo and in vitro blocking of ligand binding 7
• Other Applications Image 1 Data: Amount Used: 3 Ug/106 cells P815 cells transfected with CD80 or non-transfected P815 control cells were stained with rat anti-mouse CD80-FITC. Large cells were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
• Immunogen: Activated 5C2 cells
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate <= 1 ug/10⁶ cells
  Biotin conjugate <= 1 ug/10⁶ cells
  R-phycoerythrin conjugate <= 0.2 ug/10⁶ cells
  Allophycocyanin conjugate <= 0.2 ug/10⁶ cells
  Spectral Red conjugate <= 0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
• Uniprot ID: Q00609
• Protein Name: T-lymphocyte activation antigen CD80
• Protein Accession #: NP_033985.3?
• Nucleotide Accession #: NM_009855.2
• Tested Species Reactivity: Mouse
• Gene Symbol: CD80
• Predicted Species Reactivity: Mouse
• Application: FC
• Image 1: P815
  
  Amount Used: 3 Ug/106 cells P815 cells transfected with CD80 or non-transfected P815 control cells were stained with rat anti-mouse CD80-FITC. Large cells were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
• Image 2: DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells
  
  DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells were stained with Rat Anti-Mouse CD80-FITC.