Mouse CD25/IL-2Ra Antibody : APC (OASB00389)

• Product Number: OASB00389
• Name: Mouse CD25/IL-2Ra Antibody : APC (OASB00389)
• Clone: 7D4
• Isotype: IgM
• Conjugation: APC
• NCBI Gene Id: 16184
• Host: Rat
• Clonality: Monoclonal
• Concentration: 0.1 mg/mL
• Gene Full Name: Interleukin-2 receptor subunit alpha
• Alias Symbols: I, CD25, Il2r, Ly-4, Ly-43
• Reference: 1. Malek, T. R., R. J. Robb, and E. M. Shevach. 1983. Proc. Natl. Acad. Sci. U. S. A. 80:5694
  2. Leonard, W.J., et al. 1984. Nature 311:626
  3. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970
  4. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405
  5. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257
  6. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265
  7. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456
  8. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929
  9. Lorenzo, F., C. Jaulin, N. Vita., P. Froussard, P. Ferrara, D. Jankovic , and J. Theze. J. Immunol. 147:2970.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution for the product that is appropriate for your specific need.
• Specificity: CD25
• Additional Information: Description: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 . While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antibody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
• Application Info: Flow cytometry 1, 3, 4, 7, 8, Immunoprecipitation 1, 3
• Other Applications Image 1 Data: Amount Used: 0.1 ug/106 cells Cells from BALB/c spleen were activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours, then stained with anti-CD25-FITC and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
• Immunogen: IL-2 dependent BALB/c helper T cell clone HT2
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate <=1 ug/10⁶ cells
  Biotin conjugate <= 1 ug/10⁶ cells
  R-phycoerythrin conjugate <= 0.2 ug/10⁶ cells
  Allophycocyanin conjugate <= 0.2 ug/10⁶ cells
  Spectral Red conjugate <= 0.2 ug/10⁶ cells
  R-phycoerythrin-Cyanine 7 conjugate <= 0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert.
• Uniprot ID: P01590
• Protein Name: interleukin-2 receptor subunit alpha
• Protein Accession #: NP_032393.3?
• Nucleotide Accession #: NM_008367.3
• Tested Species Reactivity: Mouse
• Gene Symbol: IL2RA
• Predicted Species Reactivity: Mouse
• Application: FC
• Image 1: BALB/c spleen
  
  Amount Used: 0.1 ug/106 cells from BALB/c spleen were activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours, then stained with anti-CD25-FITC and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
• Image 2: Con-A stimulated BALB/c mouse splenocytes
  
  Con-A stimulated BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD25-APC.