Mouse CD4/L3T4 Antibody : FITC (OASB00259)

Data Sheet
 
Product Number OASB00259
Product Page www.avivasysbio.com/rat-anti-mouse-cd4-l3t4-antibody-fitc-oasb00259.html
Name Mouse CD4/L3T4 Antibody : FITC (OASB00259)
Clone GK1.5
Isotype IgG2b
Conjugation FITC
NCBI Gene Id 12504
Host Rat
Clonality Monoclonal
Concentration 0.5 mg/mL
Gene Full Name T-cell surface glycoprotein CD4
Alias Symbols L3T, Ly-, L3T4, Ly-4
Reference 1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller- Sieburg. 1992. Blood 60:1717
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083- 1091.
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (RPE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5 Allophycocyanin- Cyanine 5.5 (APC- CY 5.5),and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Other Applications Data Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for Mouse CD4/L3T4 Antibody : FITC (OASB00259)
Specificity CD4
Additional Information Description: CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Application Info Flow cytometry 1, Immunohistochemistry (FS) 15, Immunoprecipitation 1,2, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14
Other Applications Image 1 Data Amount Used: 0.2 Ug/106 cells Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD4-PE and anti-mouse CD8a-FITC , gated on small lymphocytes and analyzed by flow cytometry.
Immunogen Mouse CTL clone V4
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or faceprotection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physicianimmediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry: Fluorescein and Biotin conjugates <= 1 ug/106 cells
R-phycoerythrin conjugate
Allophycocyanin conjugate
R-phycoerythrin conjugate-Texas Red®
Spectral Red conjugate
Cyanine 5 conjugate
R-phycoerythrin-Cyanine 5.5 conjugate
R-phycoerythrin-Cyanine 7 conjugate
APC-CY5.5 and APC-CY7 conjugates
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of astandard reference reagent. Representative data are included in this package insert.
Uniprot ID P06332
Protein Name T-cell surface glycoprotein CD4
Protein Accession # NP_038516.1?
Nucleotide Accession # NM_013488.2
Tested Species Reactivity Mouse
Gene Symbol CD4
Predicted Species Reactivity Mouse
Application FC, IHC-F, ICC
Image 1
BALB/c mouse splenocytes
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-FITC and Rat Anti-Mouse CD8a-PE.
Image 2
BALB/c mesenteric lymph nodes
Amount Used: 0.2 Ug/106 cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD4-PE and anti-mouse CD8a-FITC, gated on small lymphocytes and analyzed by flow cytometry.
 

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