| Product Number |
OASA11733 |
| Product Page |
www.avivasysbio.com/pig-macrophages-antibody-oasa11733.html |
| Name |
PIG MACROPHAGES Antibody (OASA11733) |
| Clone |
BA4D5 |
| Isotype |
IgG2b |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Product Format |
Liquid. Phosphate buffered saline |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for PIG MACROPHAGES Antibody (OASA11733) |
| Specificity |
Clone BA4D5 recognises porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.
Some expression has also been observed on peripheral blood lymphocytes.
The antigen recognised by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.
It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognised by this antibody has not yet been fully characterised. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
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| Additional Information |
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line. |
| Additional Information |
Preservative Stabilisers: 0.09% Sodium Azide (NaN3) |
| Application Info |
Flow Cytometry: Suggested dilution: 1/50-1/100
Membrane permeabilization is required for this application. Aviva recommends the use of Leucoperm for this purpose. Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Western Blotting: Clone BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions. |
| Immunogen |
Porcine alveolar macrophages. |
| Additional Information |
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solution: Phosphate buffered saline |
| Purification |
Protein A Purified |
| Predicted Species Reactivity |
Porcine |
| Application |
Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunoprecipitation|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Pig |
| Image 1 | |
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