APOA1 Antibody (OASA09127)

Data Sheet
 
Product Number OASA09127
Product Page www.avivasysbio.com/apoa1-antibody-oasa09127.html
Name APOA1 Antibody (OASA09127)
Protein Size (# AA) 267 amino acids
Isotype Polyclonal IgG
NCBI Gene Id 335
Host Sheep
Clonality Polyclonal
Alias Symbols HPALP2, apo(a)
Product Format Purified IgG - lyophilised
Description of Target SHEEP ANTI HUMAN APOLIPOPROTEIN A1
Reconstitution and Storage Reconstitution: Use sterile DI water
Storage: Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Datasheets/Manuals Printable datasheet for anti-APOA1 antibody - OASA09127
Specificity APOLIPOPROTEIN A1
Additional Information Preparation: Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1
Preservative Stabilisers: Contains mannitol, dextran & salts
Application Info ELISA : This antibody can be used in a sandwich ELISA with OASA00955 as the capture antibody.
Protocol Information Citation: 1: Sonoyama K, Nishikawa H, Kiriyama S, Niki R. Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine. J Nutr.1995 Jan;125(1):13-9. PubMed PMID: 7815170.
Species: Rat
Experiment Name: Immunoblotting for plasma apo A-I quantification
Experiment Background: In this study, Kei et al. have collected data from the liver and examined the effects of diets containing beet fiber, cholestyramine and no fiber (fiber-free) on plasma and liver lipids and tissue apolipoprotein mRNA.
Experimental Steps: Whole plasma (0.5 uL) was subjected to 12% SDS-PAGE under reducing conditions (Laemmli 1970) and then electrophoretically transferred to nitrocellulose membrane (Hybond C extra, Amersham Inter national, Amersham, U.K.) in a semidry electroblotting apparatus (Nippon Eido, Tokyo, Japan) at constant current of 170 mA for 1 h. The blotting buffer contained 125 mmol/L Tris, 960 mmol/L glycine in 200 mL/L methanol, pH 8.3. The membrane was then incubated in a blocking solution of 50 g/L bovine serum albumin (fraction V, Miles Inc., Kankakee, IL) in Tris-buffered saline (20 mmol/L Tris-HC1, 150 mmol/L NaCl, pH 7.4), followed by the incubation for 1 h with a 1:100 dilution of the sheep anti-human apo A-I serum (Oxford, U.K.) in the blocking solution containing 500 mg/L Tween 20. The membrane was then washed three times with Tris-buffered saline containing 500 mg/L Tween 20. Next, the membrane was incubated for 1 h with a 1:2000 dilution of a second antibody of rabbit antisheep IgG conjugated with horseradish peroxidase (Jackson Immunoresearch Laboratories, West Grove, PA) in the same solution used in the incubation with the first antibody, followed by washing in the sameway with the first antibody. Identification of the antigen-antibody complex was performed using Renaissance Western Blot Chemiluminescence Reagent (DuPont NEN Research Products, Boston, MA) as recommended by the manufacturer. The relative quantity of apo A-I was estimated by scanning densitometry (Dual-Wavelength Flying-Spot Scanner CS-9000, Shimadzu, Kyoto, Japan).
Number Of Protocols: 1
Immunogen The immunogen for anti-APOA1 antibody: purified human apolipoprotein A1
Uniprot ID P02647
Protein Name Apolipoprotein A-I
Protein Accession # NP_000030.1
Gene Symbol APOA1
Predicted Species Reactivity Human
Predicted Homology Based on Immunogen Sequence Human
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