14-3-3 Antibody (OASA08966)
Data Sheet
 
Product Number OASA08966
Product Page www.avivasysbio.com/14-3-3-antibody-oasa08966.html
Product Name 14-3-3 Antibody (OASA08966)
Size 0.1mg
Protein Size (# AA) 245 amino acids
Product Format Purified IgG - liquid
Isotype Polyclonal IgG
NCBI Gene Id 443032
Host Sheep
Clonality Polyclonal
Description SHEEP ANTI 14-3-3 (pSer185)
Target Reference 1. Aitken, A. et al. (1995) 14-3-3 alpha and delta are the phosphorylated forms of Raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at Ser-Pro-Glu-Lys Motif. J. Biol. Chem. 270: 5706 - 5709.
Description of Target SHEEP ANTI 14-3-3 (pSer185)
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Specificity 14-3-3
Additional Information Peptide sequence: EILNSPEKAC
Additional Information Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
Antiserum Preparation: Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Application Info Western Blot: This product detects a band of approximately 30kDa.
* For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Lead Time Domestic: within 2-3 weeks delivery International: 2-3 weeks
Immunogen Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.
Additional Information Approx Protein Conc: IgG concentration 1.0mg/ml
Swissprot Id P29361
Protein Name 14-3-3 protein zeta/delta
Application Data Application #1: Western blot with Sheep anti-14-3-3 (pSER185) antibody - Lane 1, 2ug recombinant 14-3-3 betaLane 2, 2ug recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombi
Species Reactivity Sheep
Application WB
Predicted Homology Based on Immunogen Sequence Sheep
Image 1

Western blot with Sheep anti-14-3-3 (pSER185) antibody - Lane 1, 2ug recombinant 14-3-3 betaLane 2, 2ug recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombi
 

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