Product Number |
OASA08493 |
Product Page |
www.avivasysbio.com/vegfa-antibody-oasa08493.html |
Name |
Vegfa Antibody (OASA08493) |
Protein Size (# AA) |
392 amino acids |
Isotype |
Polyclonal IgG |
NCBI Gene Id |
22339 |
Host |
Rabbit |
Clonality |
Polyclonal |
Alias Symbols |
V, Veg, Vpf, Vegf, VEGF12, VEGF16, VEGF18 |
Product Format |
Purified IgG - lyophilised |
Description of Target |
RABBIT ANTI MOUSE VEGF
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Reconstitution and Storage |
Reconstitution: Reconstitute with 0.1 ml distilled water Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide |
Datasheets/Manuals |
Printable datasheet for anti-Vegfa antibody - OASA08493 |
Specificity |
OASA08493 is specific for mouse Vascular Endothelial Growth Factor (VEGF) VEGF is a homodimeric protein expressed by various vascularized tissues. VEGF is reported to stimulate endothelial cell growth and angiogenesis.
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Additional Information |
Note: Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use. For long term |
Additional Information |
Preservative Stabilisers: None present Antiserum Preparation: Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Application Info |
Western Blotting : 0.1ug/ml - 0.2ug/ml Immunofluorescence: 2.5ug/ml ELISA : 0.5ug/ml This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with OASA08494 as a detection reagent and OPSA11123 as the standard. Immunohistology - Paraffin: 2.5ug/ml This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose. Histology Positive Control Tissue : Kidney |
Immunogen |
The immunogen for anti-Vegfa antibody: recombinant mouse VEGF |
Additional Information |
Approx Protein Conc: IgG concentration 1.0 mg/ml after reconstitution. Buffer Solutions: Phosphate buffered saline |
Uniprot ID |
Q00731 |
Protein Name |
Vascular endothelial growth factor A |
Protein Accession # |
NP_001020421.2 |
Gene Symbol |
Vegfa |
Predicted Species Reactivity |
Mouse |
Application |
WB, IHC-P, IF |
Predicted Homology Based on Immunogen Sequence |
Mouse |
Image 1 | |
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