Vegfa Antibody (OASA08493)

Data Sheet
 
Product Number OASA08493
Product Page www.avivasysbio.com/vegfa-antibody-oasa08493.html
Name Vegfa Antibody (OASA08493)
Protein Size (# AA) 392 amino acids
Isotype Polyclonal IgG
NCBI Gene Id 22339
Host Rabbit
Clonality Polyclonal
Alias Symbols V, Veg, Vpf, Vegf, VEGF12, VEGF16, VEGF18
Product Format Purified IgG - lyophilised
Description of Target RABBIT ANTI MOUSE VEGF
Reconstitution and Storage Reconstitution: Reconstitute with 0.1 ml distilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Datasheets/Manuals Printable datasheet for anti-Vegfa antibody - OASA08493
Specificity OASA08493 is specific for mouse Vascular Endothelial Growth Factor (VEGF)
VEGF is a homodimeric protein expressed by various vascularized tissues.
VEGF is reported to stimulate endothelial cell growth and angiogenesis.
Additional Information Note: Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
For long term
Additional Information Preservative Stabilisers: None present
Antiserum Preparation: Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Application Info Western Blotting : 0.1ug/ml - 0.2ug/ml
Immunofluorescence: 2.5ug/ml
ELISA : 0.5ug/ml
This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with OASA08494 as a detection reagent and OPSA11123 as the standard.
Immunohistology - Paraffin: 2.5ug/ml
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Histology Positive Control Tissue : Kidney
Immunogen The immunogen for anti-Vegfa antibody: recombinant mouse VEGF
Additional Information Approx Protein Conc: IgG concentration 1.0 mg/ml after reconstitution.
Buffer Solutions: Phosphate buffered saline
Uniprot ID Q00731
Protein Name Vascular endothelial growth factor A
Protein Accession # NP_001020421.2
Gene Symbol Vegfa
Predicted Species Reactivity Mouse
Application WB, IHC-P, IF
Predicted Homology Based on Immunogen Sequence Mouse
Image 1

 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com