CCL2 Antibody (OASA07962)

• Product Number: OASA07962
• Name: CCL2 Antibody (OASA07962)
• Protein Size (# AA): 99 amino acids
• Isotype: Polyclonal IgG
• NCBI Gene Id: 6347
• Host: Rabbit
• Clonality: Polyclonal
• Alias Symbols: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30
• Product Format: Purified IgG - lyophilised
• Reference: 1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination. J. Neuroinflammation. 2: 7-20.
  
• Description of Target: RABBIT ANTI HUMAN MCP-1
  
• Reconstitution and Storage: Reconstitution: Reconstitute with 0.1 ml distilled water.
  For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
  Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.
  This product should be stored undiluted.
  Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  
• Specificity: MCP-1
• Additional Information: Preservative Stabilisers: None present
  Antiserum Preparation: Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Application Info: ELISA:    0.5ug/ml
  Western Blotting:    0.1ug/ml - 0.2ug/ml
  Functional Assays:    2.0ug/ml - 3.0ug/ml
  following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
   
  Yes
  No
  Not Determined
  Suggested DilutionFlow Cytometry
   
   
  
   Immunohistology - Frozen
   
   
  
   Immunohistology - Paraffin
   
   
  
   Immunohistology - Resin
   
   
  
   ELISA
  
   
   
  0.5ug/mlImmunoprecipitation
   
   
  
   Western Blotting
  
   
   
  0.1ug/ml - 0.2ug/mlFunctional Assays
  
   
   
  2.0ug/ml - 3.0ug/ml
  Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using apropriate negative/positive controls.
• Immunogen: The immunogen for anti-CCL2 antibody: recombinant human MCP-1
• Additional Information: Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
• Uniprot ID: P13500
• Protein Name: C-C motif chemokine 2
• Protein Accession #: NP_002973.1
• Gene Symbol: CCL2
• Predicted Species Reactivity: Human
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: