Product Number |
OASA05048 |
Product Page |
www.avivasysbio.com/mrc1-antibody-oasa05048.html |
Name |
Mrc1 Antibody (OASA05048) |
Molecular Weight |
175 kDa |
Clone |
MR5D3 |
Isotype |
IgG2a |
NCBI Gene Id |
17533 |
Host |
Rat |
Clonality |
Monoclonal |
Concentration |
1 mg/ml |
Description |
|
Alias Symbols |
M, MR, CD206, AW259686 |
Product Format |
Liquid. Phosphate buffered saline |
Description of Target |
CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins.
Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in Western Blot. |
Reconstitution and Storage |
-20°C |
Datasheets/Manuals |
Printable datasheet for Mrc1 Antibody (OASA05048) |
Specificity |
Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175 kDa type 1 membrane glycoprotein that is also known as CD206. |
Additional Information |
Fusion Partners: Spleen cells from immunized Fischer rats were fused with cells of the Y3 myeloma cell line. Carrier Free: Yes |
Application Info |
FC: 1/10-1/20 (Use 10 uL of the suggested working dilution to label 106 cells in 100 uL.) CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
Optimal dilutions should be determined by the end user.
|
Immunogen |
Chimaeric CRD4-7-Fc protein |
Uniprot ID |
Q61830 |
Protein Name |
Macrophage mannose receptor 1 |
Publications |
Cathepsin B-Mediated Activation of Trypsinogen in Endocytosing Macrophages Increases Severity of Pancreatitis in Mice. Gastroenterology. 154, 704-718.e10 (2018). 29079517
Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol. 1, 678-694 (2015). 26693174
NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 158, 253-269.e14 (2020). 31593700 |
Protein Accession # |
NP_032651.2 |
Purification |
Protein G Purified |
Nucleotide Accession # |
NM_005625 |
Gene Symbol |
Mrc1 |
Predicted Species Reactivity |
Mouse |
Application |
Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunoprecipitation |
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