| Product Number |
OASA02910 |
| Product Page |
www.avivasysbio.com/hras-antibody-oasa02910.html |
| Name |
HRAS Antibody (OASA02910) |
| Protein Size (# AA) |
189 amino acids |
| Clone |
H-Ras-03 |
| Isotype |
IgG1 |
| NCBI Gene Id |
3265 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Alias Symbols |
CTLO, HAMSV, HRAS1, RASH1, p21ras, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1 |
| Product Format |
Liquid. Phosphate buffered saline |
| Reference |
1. Zheng,Y. and Quilliam, L.A. (2003) Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO reports. 4: 463-468.
|
| Description of Target |
MOUSE ANTI HUMAN H-Ras
|
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for HRAS Antibody (OASA02910) |
| Specificity |
Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburn et al. 1990).
H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006).
Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluoto et al. 2011). |
| Additional Information |
Preservative Stabilisers: 0.09% Sodium Azide (NaN3) |
| Additional Information |
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline |
| Application Info |
Western Blot: This product detects a band of approximately 24kDa in HeLa, K567 and RAJI whole cell lysates under reducing conditions.
Suggested Dilution: 0.5-1.0 ug/ml |
| Immunogen |
Synthetic peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras. |
| Uniprot ID |
P01112 |
| Protein Name |
GTPase HRas |
| Protein Accession # |
NP_001123914.1 |
| Purification |
Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Gene Symbol |
HRAS |
| Predicted Species Reactivity |
Human |
| Application |
Enzyme-linked immunosorbent assay|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | |
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