Anti-Collagen Type I Antibody (OARA02579)

Data Sheet
 
Product Number OARA02579
Product Page www.avivasysbio.com/anti-collagen-type-i-antibody-oara02579.html
Name Anti-Collagen Type I Antibody (OARA02579)
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 1277
Host Rabbit
Clonality Polyclonal
Concentration Varies by lot. See vial for concentration.
Gene Full Name collagen type I alpha 1 chain
Alias Symbols alpha-1 type I collagen;alpha1(I) procollagen;CAFYD;collagen alpha 1 chain type I;collagen alpha-1(I) chain;collagen alpha-1(I) chain preproprotein;collagen of skin, tendon and bone, alpha-1 chain;collagen, type I, alpha 1;EDSARTH1;EDSC;OI1;OI2;OI3;OI4;pro-alpha-1 collagen type 1;type I proalpha 1;type I procollagen alpha 1 chain.
Product Format Liquid 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide
Description of Target Type I collagen is a member of group I collagen (fibrillar forming collagen).
Reconstitution and Storage Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Datasheets/Manuals Printable datasheet for Anti-Collagen Type I Antibody (OARA02579)
Specificity Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Application Info Anti-Collagen Type I has been tested by Western blot, dot blot, and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, IP, native PAGE, immunofluorescence, and FC for highly sensitive qualitative analysis.
Immunogen Collagen Type I from human and bovine placenta
Dilution ELISA: 1:5000~50000
FLISA: 1:100
IHC: 1:50~200
IP: 1:100
Uniprot ID P02452
Protein Name Collagen alpha-1(I) chain|collagen alpha-1(I) chain preproprotein [Homo sapiens]
Protein Accession # https://www.ncbi.nlm.nih.gov/protein/NP_000079.2
Purification COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.
Gene Symbol COL1A1
Predicted Species Reactivity Human, Mouse, Rat, Bovine, Pig
Application Dot blot|Enzyme-linked immunosorbent assay|Flow cytometry|Fluorescence-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunoprecipitation|Multiplex assay|Western blot
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