| Product Number |
OARA02141 |
| Product Page |
www.avivasysbio.com/msln-antibody-oara02141.html |
| Name |
MSLN Antibody (OARA02141) |
| Clone |
MB-G10 |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
10232 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
mesothelin |
| Alias Symbols |
CAK1 antigen;megakaryocyte potentiating factor;mesothelin;MPF;pre-pro-megakaryocyte-potentiating factor;SMRP;soluble MPF mesothelin related protein. |
| Product Format |
Liquid (sterile filtered) |
| Reference |
Chang K, Pastan I, Willingham MC. (1992) Isolation and characterization of a monoclonal antibody reactive with ovarian cancers and normal mesothelium. Int J Cancer 50:373-81.Argani P, Iacobuzio-Danahue C, Ryu B, et al. (2001) Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas: identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res 7:3862-8.Chang K, Pastan I.(1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesothelioma, and ovarian cancers. Proc Natl Acad Sci U S A 93:136-40. |
| Description of Target |
Membrane-anchored forms may play a role in cellular adhesion. |
| Reconstitution and Storage |
-20°C|See manual |
| Datasheets/Manuals |
Printable datasheet for MSLN Antibody (OARA02141) |
| Specificity |
This antibody is directed against human mesothelin protein. Cross reactivity with homologues from other sources has not been tested. |
| Additional Information |
Label: Unconjugated
Stabilizer: None |
| Application Info |
ELISA: 1:10,000 - 1:50,000
WB: 1:1,000
IHC: 1:100 |
| Immunogen |
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. |
| Uniprot ID |
Q13421 |
| Protein Name |
Mesothelin |
| Protein Accession # |
NP_001170826.1; NP_005814.2; NP_037536.2; XP_005255091.1; XP_006720900.1 |
| Purification |
This product was purified from tissue culture supernatant fluid by Protein A chromatography. |
| Application Data |
This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature. |
| Nucleotide Accession # |
NM_001177355.1; NM_005823.5; NM_013404.4; XM_005255034.3; XM_006720837.2 |
| Gene Symbol |
MSLN |
| Predicted Species Reactivity |
Human |
| Application |
Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 |
 | SDS-PAGE of Mouse anti-Mesothelin Monoclonal Antibody. Lane 1: Non-Reduced Mouse anti-Mesothelin Monoclonal Antibody
Lane 2: 3 uL OPAL Pre-stained Marker (p/n MB-210-0500).
Lane3: Reduced Mouse anti-Mesothelin Monoclonal Antibody. Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa. |
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