GLRA1 Antibody (OAPC00117)

• Product Number: OAPC00117
• Name: GLRA1 Antibody (OAPC00117)
• Molecular Weight: ~48kDa
• NCBI Gene Id: 25674; 24397
• Host: Rabbit
• Clonality: Polyclonal
• Alias Symbols: GLYRA1
• Product Format: Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
• Reference: Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964.
  Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798.
  Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220.
  Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, Wässle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648.
  Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389.
  Schofield CM, Jenkins A, Harrison NL (2003) A highly conserved aspartic acid residue in the signature disulfide loop of the alpha 1-subunit is a determinant of gating in the glycine receptor. J Biol Chem 278:34079-34083.
• Description of Target: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
• Reconstitution and Storage: Packaging: 200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate.Adequate amount of material to conduct 10-mini Western Blots.
  Reconstitution: The antibody should be reconstituted in 100 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2HPO4, pH 7.4) before use.
  Storage: After reconstitution the antibody should be aliquoted and stored at -20C.
• Specificity: Specific for the ~48k alpha 1- and alpha 2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts containing the recombinant human Glycine Receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the alpha1 subunit. Does not recognize other glycine receptor subunits.
• Application Info: The antibody has been directly tested for reactivity in Western blots in human, mouse and rat tissues.
  Western blot of rat spinal cord showing specific immunolabeling of the ~48k alpha 1- and alpha 2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.
  Working Dilutions:
  DB~~1:1000
  IHC~~1:1000
  WB~~ 1:1000
• Immunogen: Peptide from the N-terminus region of the alpha 1-subunit of rat glycine receptor.
• Uniprot ID: P07727, P22771
• Protein Name: Glycine receptor subunit alpha-1, Glycine receptor subunit alpha-2
• Publications:

  Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction. Eur. J. Pharmacol. 762, 82-8 (2015). 26004535

• Protein Accession #: NP_037265.1, NP_036700.1
• Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
• Gene Symbol: GLRA1, GLRA2
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, DB
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat
• Image 1: Rat spinal cord
  
  Western blot of rat spinal cord showing specific immunolabeling of the ~48k alpha 1- and alpha 2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.