Anti-Glial Fibrillary Acidic Protein (GFAP) (OAPC00115)

• Product Number: OAPC00115
• Name: Anti-Glial Fibrillary Acidic Protein (GFAP) (OAPC00115)
• Molecular Weight: ~50kDa
• NCBI Gene Id: 281189
• Host: Chicken
• Clonality: Polyclonal
• Product Format: Liquid. 10mM sodium azide.
• Reference: 1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
  2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001)
• Description of Target: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
• Specificity: Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
• Application Info: The antibody has been directly tested for reactivity in human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.
  Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
• Immunogen: Recombinant and purified bovine GFAP.
• Uniprot ID: Q28115
• Protein Name: Glial fibrillary acidic protein
• Protein Accession #: NP_776490.2
• Purification: Total IgY fraction.
• Gene Symbol: GFAP
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IF
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: Rat Cortex
  
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.
• Image 2: neurons and glia
  
  Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.