Anti-Alpha-Internexin (NF66) (OAPC00002)

• Product Number: OAPC00002
• Name: Anti-Alpha-Internexin (NF66) (OAPC00002)
• Molecular Weight: ~66kDa
• Clone: 1D2
• Isotype: IgG¹
• NCBI Gene Id: 24503
• Host: Mouse
• Clonality: Monoclonal
• Alias Symbols: Nf66, Inexa, Intlaa, alpha-Inx
• Product Format: Liquid containing 10 mM sodium azide.
• Reference: 1. Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).
  2. McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002).
  3. Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004).
  4. Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004).
  5. Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).
• Description of Target: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
• Specificity: Specific for the ~66k alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.
• Additional Information: Preservative:10mM sodium azide.
• Application Info: The antibody has been directly tested for reactivity in a wide variety of mammalian species.
  Immunostaining of cultured rat CNS cells showing specific labeling of alpha-internexin in neuronal processes in red and anti-chicken MAP2 antibody (cat. # OAPC00006) in green.
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.
  Suggested dilutions are:
  WB:1:2000
  IF:1:250
  IHC:1:1000
• Immunogen: Recombinant rat alpha-internexin expressed in and purified from E. coli.
• Uniprot ID: P23565
• Protein Name: Alpha-internexin
• Protein Accession #: NP_062001.1
• Purification: Total IgG fraction
• Gene Symbol: INA
• Predicted Species Reactivity: Most mammals
• Application: WB, IF, IHC
• Predicted Homology Based on Immunogen Sequence: Most mammals
• Image 1: Rat cortex
  
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.
• Image 2: Rat CNS
  
  Immunostaining of cultured rat CNS cells showing specific labeling of alpha-internexin in neuronal processes in red and anti-chicken MAP2 antibody (cat. # OAPC00006) in green.