H1N1 Hemagglutinin Antibody (OAPB00912)

• Product Number: OAPB00912
• Name: H1N1 Hemagglutinin Antibody (OAPB00912)
• Conjugation: Unconjugated
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Hemagglutinin
• Product Format: Liquid. Seasonal H1N1 Hemagglutinin Antibody is supplied in PBS containing 0.02% sodium azide.
• Reference: Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA2003; 289:179-186.;Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, et al. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N. Engl. J. Med.2009; 360:2605-15.;Butler D. Swine flu goes global. Nature2009; 458:1082-3.;Morens DM, Taubenberger JK, and Fauci AS. The Persistent Legacy of the 1918 Influenza Virus. N. Engl. J. Med.2009; Jun 29.
• Description of Target: Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
• Reconstitution and Storage: Seasonal H1N1 Hemagglutinin antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Additional Information: Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Application Info: Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H1N1 strain of common influenza A in ELISA. It will detect 2 ng of free peptide at 1 ug/ml and will not cross-react with peptide corresponding to the swine-origin H1N1 influenza Hemagglutinin. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 ug/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/ml.
• Blocking Peptide: For anti-H1N1 Hemagglutinin (OAPB00912) antibody is Catalog # OEPB02977
• Immunogen: Hemagglutinin antibody was raised against a synthetic peptide from the Hemagglutinin protein. The peptide sequence is unique from the peptide sequence for product 5231 and 5239. This antibody is a cognate pair with antibody 5237. The immunogen is located within amino acids 180 - 230 of Seasonal H1N1 Hemagglutinin.
• Uniprot ID: Q67010
• Protein Name: Hemagglutinin|hemagglutinin [Influenza A virus (A/Brisbane/59/2007(H1N1))]
• Publications:

  Huang, H. et al. NF-κB1 inhibits c-Myc protein degradation through suppression of FBW7 expression. Oncotarget 5, 493-505 (2014). 24457827

• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/ACA28844
• Purification: Affinity Purified
• Gene Symbol: H1N1 Hemagglutinin
• Predicted Species Reactivity: Influenza A Virus|Virus
• Application: Enzyme-linked immunosorbent assay
• Predicted Homology Based on Immunogen Sequence: Virus
• Image 1: Hemagglutinin
  
  Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A & B) and swine-origin Hemagglutinin (lanes C & D) with anti-seasonal Hemagglutinin antibody (5235) at 2 ug/mL (lanes A & C) and anti-swine-origin Hemagglutinin antibody (5237) at 2 ug/mL (lanes B & D).
• Image 2:
  
  ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 ug/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/ml.