ERBB2 Antibody (OAPB00881)

• Product Number: OAPB00881
• Name: ERBB2 Antibody (OAPB00881)
• Isotype: IgY
• Conjugation: Unconjugated
• NCBI Gene Id: 2064
• Host: chicken
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: erb-b2 receptor tyrosine kinase 2
• Alias Symbols: CD340;c-erb B2/neu protein;HER2;HER-2;HER-2/neu;herstatin;human epidermal growth factor receptor 2;metastatic lymph node gene 19 protein;MLN 19;NEU;neuro/glioblastoma derived oncogene homolog;neuroblastoma/glioblastoma derived oncogene homolog;NGL;p185erbB2;proto-oncogene c-ErbB-2;proto-oncogene Neu;receptor tyrosine-protein kinase erbB-2;TKR1;tyrosine kinase-type cell surface receptor HER2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2;v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;VSCN2.
• Product Format: Liquid. erbB-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Reference: Baselga J and Swain SM. Novel anticancer targets: revisiting ERBB2 and discovering ERBB3. Nature Rev.2009; 9:463-475.;Slamon DJ, Clark GM, Wong SG, et al. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science1987; 235:177-82.;Vermeij J, Teugels E, Bourgain C, et al. Genomic activation of the EGFR and HER2-neu genes in a significant proportion of invasive epithelial ovarian cancers. BMC Cancer2008; 8:3.;Marmor MD, Skaria KB, and Yarden Y. Signal transduction and oncogenesis by ErbB/HER receptors. Int. J. Rad. Oncol. Biol. Phys.2004; 58:903-13.
• Description of Target: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Reconstitution and Storage: erbB-2 antibody can be stored at 4C for three months and -20C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Additional Information: Positive Controls: Rat Liver Tissue Lysate
• Application Info: WB: 1-2 ug/mL
  IHC-P: 2.5 ug/mL
  IF: 20 ug/mL
• Blocking Peptide: For anti-ERBB2 (OAPB00881) antibody is Catalog # OEPB03003
• Immunogen: erbB-2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human erbB-2. The immunogen is located within the last 50 amino acids of erbB-2.
• Uniprot ID: P04626
• Protein Name: Receptor tyrosine-protein kinase erbB-2|receptor tyrosine-protein kinase erbB-2 isoform a precursor [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004439
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001005862.2; NM_001289936.1; NM_001289938.1; NM_004448.3
• Gene Symbol: ERBB2
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Image 1: rat liver
  
  Western blot analysis of erbB-2 in rat liver tissue lysate with erbB-2 antibody at (A) 1 and (B) 2 ug/mL.
• Image 2: human liver
  
  Immunohistochemistry of erbB-2 in human liver tissue with erbB-2 antibody at 2.5 ug/mL.