Aak1 Antibody (OAPB00768)

• Product Number: OAPB00768
• Name: Aak1 Antibody (OAPB00768)
• Conjugation: Unconjugated
• NCBI Gene Id: 22848
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: AP2 associated kinase 1
• Alias Symbols: adaptor-associated kinase 1;AP2-associated protein kinase 1.
• Product Format: Liquid. Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Reference: Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9.;Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep.2003; 4:246-51.;Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol.2002; 156:791-5.;Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell2007; 18:2698-706.
• Description of Target: Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
• Reconstitution and Storage: Aak1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Additional Information: Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Application Info: Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 ug/mL.
• Blocking Peptide: For anti-Aak1 antibody is Catalog # OEPB02885
• Immunogen: Aak1 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1. The immunogen is located within amino acids 190 - 240 of Aak1.
• Uniprot ID: Q2M2I8
• Protein Name: AP2-associated protein kinase 1|AP2-associated protein kinase 1 [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_055726
• Purification: Affinity Purified
• Gene Symbol: AAK1
• Predicted Species Reactivity: Bovine|Human|Mouse|Porcine|Rat
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: A-20
  
  Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL.
• Image 2: A-20
  
  Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 ug/mL.