| Product Number |
OAPB00726 |
| Product Page |
www.avivasysbio.com/asah1-antibody-oapb00726.html |
| Name |
ASAH1 Antibody (OAPB00726) |
| Molecular Weight |
Predicted: 45 kDa Observed: 49 kDa |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
427 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
N-acylsphingosine amidohydrolase 1 |
| Description |
|
| Alias Symbols |
AC;ACDase;acid CDase;acid ceramidase;acylsphingosine deacylase;ASAH;N-acylethanolamine hydrolase ASAH1;N-acylsphingosine amidohydrolase;N-acylsphingosine amidohydrolase (acid ceramidase) 1;PHP;PHP32;putative 32 kDa heart protein;SMAPME. |
| Product Format |
Liquid. ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Description of Target |
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. |
| Reconstitution and Storage |
Can be stored at 4°C for three months and -20°C stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Datasheets/Manuals |
Printable datasheet for ASAH1 Antibody (OAPB00726) (OAPB00726) |
| Specificity |
Multiple isoforms of ASAH1 are known to exist. |
| Additional Information |
Positive Control: Human heart tissue |
| Application Info |
WB: 1-2 ug/mL
IHC-P: 2.5 ug/mL
IF: 20 ug/mL
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Optimal dilutions should be determined by the end user.
|
| Immunogen |
ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1. |
| Uniprot ID |
Q13510 |
| Protein Name |
Acid ceramidase|N-acylsphingosine amidohydrolase (acid ceramidase) 1, isoform CRA_c [Homo sapiens] |
| Publications |
Increased placental mitochondrial fusion in gestational diabetes mellitus: an adaptive mechanism to optimize feto-placental metabolic homeostasis?. BMJ Open Diabetes Res Care. 8, (2020). 32144130 |
| Protein Accession # |
https://www.ncbi.nlm.nih.gov/protein/EAW63795 |
| Purification |
Affinity Purified |
| Gene Symbol |
ASAH1 |
| Predicted Species Reactivity |
Bovine|Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Bovine (88%) |
| Image 1 | Mouse Heart
 | Western blot analysis of ASAH1 in mouse heart tissue lysate with ASAH1 antibody at (A) 1 and (B) 2 ug/mL. |
|
| Image 2 | human heart
 | Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 ug/mL. |
|
