4E-BP1 Antibody (OAPB00309)

• Product Number: OAPB00309
• Name: 4E-BP1 Antibody (OAPB00309)
• Conjugation: Unconjugated
• NCBI Gene Id: 1978
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Alias Symbols: 4EBP1;4E-BP1;BP-1;eIF4E-binding protein 1;eukaryotic translation initiation factor 4E-binding protein 1;PHAS-I;phosphorylated heat- and acid-stable protein regulated by insulin 1.
• Product Format: Liquid. 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Reference: Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2000; 279:E715-29.;Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.;
• Description of Target: Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
• Reconstitution and Storage: 4E-BP1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Additional Information: Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Application Info: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2. 5 - 5 ug/ml. (Optimal dilution should be determined by user)
• Blocking Peptide: For anti-4E-BP1 antibody is Catalog # OEPB02402
• Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1.
• Uniprot ID: Q13541
• Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1|eukaryotic translation initiation factor 4E-binding protein 1 [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004086
• Purification: Affinity Purified
• Gene Symbol: EIF4EBP1
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: 3T3
  
  Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
• Image 2: 3T3
  
  Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 μg/mL.