Bcl-2 Antibody : HRP (OAPB00242-HRP)

Data Sheet
 
Product Number OAPB00242-HRP
Product Page www.avivasysbio.com/bcl-2-antibody-hrp-oapb00242-hrp.html
Name Bcl-2 Antibody : HRP (OAPB00242-HRP)
Isotype IgG
Conjugation HRP: Horseradish Peroxidase
NCBI Gene Id 596
Host Rabbit
Clonality Polyclonal
Concentration 0.6-0.7 mg/ml
Gene Full Name B-cell CLL/lymphoma 2
Alias Symbols Bcl-2, PPP1R50
Product Format Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reference Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.;
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.;
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.;
Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
Description of Target (NT) a member of the bcl-2 family
Reconstitution and Storage All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Datasheets/Manuals Printable datasheet for anti-Bcl-2 antibody - OAPB00242-HRP
Application Info Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 4 ug/ml. Bcl-2 antibody can also detect Bcl-2 by immunohistochemistry at 2 ug/ml. For immunofluorescence start at 10 ug/ml. (Optimal dilution should be determined by user)
Blocking Peptide For anti-Bcl-2 antibody is Catalog # OEPB02336
Immunogen The immunogen for anti-Bcl-2 antibody: antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-2.
Protein Accession # AAH27258
Purification Ion exchange chromatography purified
Gene Symbol BCL2
Predicted Species Reactivity Human, Mouse
Application WB, IHC, IF, ELISA
Predicted Homology Based on Immunogen Sequence Human; Mouse
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