| Product Number |
OAPB00242 |
| Product Page |
www.avivasysbio.com/bcl-2-antibody-oapb00242.html |
| Name |
Bcl-2 Antibody (OAPB00242) |
| Molecular Weight |
Predicted: 26kD Observed: 26 kD |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
596 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Varies by lot. se |
| Gene Full Name |
BCL2 apoptosis regulator |
| Description |
|
| Alias Symbols |
apoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;PPP1R50;protein phosphatase 1, regulatory subunit 50. |
| Product Format |
Liquid PBS containing 0.02% sodium azide. |
| Reference |
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.;
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.;
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.;
Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6. |
| Description of Target |
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). |
| Reconstitution and Storage |
Bcl-2antibody can be stored at 4°C, for three months and -20°C stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Datasheets/Manuals |
Printable datasheet for Bcl-2 Antibody (OAPB00242) (OAPB00242) |
| Application Info |
WB: 1-2 ug/mL
IHC-P: 2 ug/mL
IF: 10 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples ; Immunofluorescence in human and mouse samples. |
| Blocking Peptide |
For anti-Bcl-2 antibody is Catalog # OEPB02336 |
| Immunogen |
Anti-Bcl-2 antibody (3335) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2. |
| Uniprot ID |
P10415 |
| Protein Name |
Apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2 [Homo sapiens] |
| Publications |
Age-Related Gliosis Promotes Central Nervous System Lymphoma through CCL19-Mediated Tumor Cell Retention. Cancer Cell. 36, 250-267.e9 (2019). 31526758 |
| Protein Accession # |
https://www.ncbi.nlm.nih.gov/protein/AAH27258 |
| Purification |
Ion Exchange |
| Gene Symbol |
BCL2 |
| Predicted Species Reactivity |
Bovine|Chicken|Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Bovine (100%), Rat (100%), Chicken (93%) |
| Image 1 | Daudi
 | Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 ?g/mL. |
|
| Image 2 | Human Kidney
 | Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 ?g/mL. |
|
| Image 3 | Human Kidney
 | Immunofluorescence of Bcl-2 in Human Kidney cells with Bcl-2 antibody at 10 ug/mL. |
|
