ACE2 Antibody (OAPB00220)

• Product Number: OAPB00220
• Name: ACE2 Antibody (OAPB00220)
• Molecular Weight: Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation)
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 59272
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: angiotensin converting enzyme 2
• Alias Symbols: ACEH;ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2;angiotensin-converting enzyme homolog;angiotensin-converting enzyme-related carboxypeptidase;metalloprotease MPROT15;peptidyl-dipeptidase A;truncated angiotensin converting enzyme 2.
• Product Format: Liquid. ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Reference: Donoghue et al. Circ. Res. 2000;87:1-9.
  Tipnis et al. J Biol. Chem. 2000;275:33238-43.
  Li et al. Nature 2003;426:450-4.
  Lu et al. The Lancet 2020 (published online).
  Crackower et al. Nature 2002;417:822-8.
• Description of Target: Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).
• Reconstitution and Storage: Stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Specificity: Anti-ACE2 has no cross response to ACE1.
• Additional Information: Positive Control: Human Kidney Tissue Lysate, Human Kidney Tissue Slide
• Additional Information: Isoforms: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kDa) and isoform 2 (555aa, 64kDa). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kDa) and isoform 2 (353aa, 40kDa). Rat ACE2 has one isoform (805aa, 93kDa). This product can detect human, mouse and rat.
• Application Info: WB: 4 ug/mL. IHC: 2 ug/mL. IF: 20 ug/mL.
  Antibody validated: Western Blot in human, mouse and rat samples. Immunohistochemistry in human, mouse and rat samples. Immunofluorescence in human samples. All other applications and species not yet tested.
• Blocking Peptide: For anti-ACE2 antibody is Catalog # OEPB02314
• Immunogen: ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2. The immunogen is located within amino acids 150 - 200 of ACE2.
• Uniprot ID: Q9BYF1
• Protein Name: Angiotensin-converting enzyme 2|angiotensin-converting enzyme 2 precursor [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_068576
• Purification: Affinity Purified
• Gene Symbol: ACE2
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Predicted Homology Based on Immunogen Sequence: Predicted species reactivity based on immunogen sequence: Bovine: (93%)
• Image 1: Human Kidney Cells
  
  Figure 5 Immunofluorescence Validation of ACE2 in Human Kidney Cells
  Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
• Image 2: Human Kidney Tissue
  
  Figure 4 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue
  Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
• Image 3: Human Tissues
  
  Figure 1 Western Blot Validation in Human Tissues
  Loading: 15 ug of lysates per lane. Antibodies: ACE2 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
• Image 4: Mouse Stomach Tissue
  
  Figure 2 Western Blot Validation in Mouse Stomach Tissue
  Loading: 15 ug of lysates per lane. Antibodies: ACE2 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
• Image 5: Rat Brain Tissue
  
  Figure 3 Western Blot Validation in Rat Brain Tissue
  Loading: 15 ug of lysates per lane. Antibodies: ACE2 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.