| Product Number |
OALA02443 |
| Product Page |
www.avivasysbio.com/adora2a-antibody-oala02443.html |
| Name |
ADORA2A Antibody (OALA02443) |
| Clone |
7F6-G5-A2 / 7F6 |
| Isotype |
IgG2a |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
135 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
0.5 mg/ml |
| Protein Range |
3rd Intracellular Domain |
| Description |
|
| Alias Symbols |
ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8, AA2AR, A2AR |
| Product Format |
Liquid0.01M Tris-Glycine (pH 7.4), 0.15M sodium chloride, 0.05% sodium azide |
| Reconstitution and Storage |
Short term: store at 4C. Long term: aliquot and store at -20C. Avoid freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OALA02443 |
| Specificity |
Recognizes human adenosine receptor A2A at ~43kD. |
| Additional Information |
Antigen Type: Recombinant Protein |
| Application Info |
FC, IHC, IHC-P (5 ug/ml), IHC-PFA (1 ug/ml), WB (1:1000) |
| Immunogen |
6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. |
| Warning |
For research use only. |
| Epitope |
3rd Intracellular Domain |
| Storage Buffer |
0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide |
| Uniprot ID |
P29274 |
| Publications |
Toll-like receptor 2 induces adenosine receptor A2a and promotes human squamous carcinoma cell growth via extracellular signal regulated kinases ½. Oncotarget. 9, 6814-6829 (2018). 29467931 |
| Protein Accession # |
NP_000666.2 |
| Purification |
Protein G purified |
| Application Data |
Immunohistochemistry: OALA02443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02443 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras. |
| Nucleotide Accession # |
NM_000675 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
ADORA2A |
| Predicted Species Reactivity |
Rabbit, Mouse, Dog, Guinea pig, Rat, Human |
| Application |
FC, IHC, IHC-P, IHC-PFA, WB |
| Image 1 | |
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