| Product Number |
OAGA08528 |
| Product Page |
www.avivasysbio.com/slc16a1-antibody-oaga08528.html |
| Name |
SLC16A1 Antibody (OAGA08528) |
| Clone |
GT14612 |
| Isotype |
IgG2b |
| NCBI Gene Id |
6566 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
solute carrier family 16 member 1 |
| Alias Symbols |
MCT, HHF7, MCT1, MCT1D |
| Product Format |
Liquid |
| Description of Target |
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAGA08528 |
| Application Info |
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MCT1. The exact sequence is proprietary. |
| Storage Buffer |
PBS |
| Uniprot ID |
P53985 |
| Protein Name |
monocarboxylate transporter 1 |
| Protein Accession # |
NP_001159968.1 |
| Purification |
Affinity purified by Protein G. |
| Nucleotide Accession # |
NM_001166496.1 |
| Gene Symbol |
SLC16A1 |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, IHC-P, WB |
| Image 1 | THP-1 cells
 | MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCT1 protein stained by MCT1 antibody [GT14612] diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um. |
| | Image 2 | Human colon carcinoma
 | MCT1 antibody [GT14612] detects MCT1 protein at weak membrane and strong cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
MCT1 antibody [GT14612] dilution: 1:500.
|
| | Image 3 | whole cell
 | MCT1 antibody detects MCT1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
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