Product Number |
OAGA08352 |
Product Page |
www.avivasysbio.com/6x-his-tag-antibody-oaga08352.html |
Name |
6X His tag Antibody (OAGA08352) |
Clone |
GT161 |
Isotype |
IgG2a |
Host |
Mouse |
Clonality |
Monoclonal |
Concentration |
1 mg/ml |
Product Format |
Liquid |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAGA08352 |
Application Info |
Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Storage Buffer |
PBS |
Purification |
Affinity purified by Protein G. |
Gene Symbol |
6X His tag |
Application |
WB |
Image 1 | His-tagged recombinant proteins
| 6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/ml 6X His tag antibody [GT161] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates. |
| Image 2 | E. coli
| 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT161] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
| Image 3 | E. coli
| 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT161] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
| Image 4 | GST-recombinant protein
| 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. 2 ug GST-recombinant protein B. 2 ug His-recombinant protein C. 1 ug His-recombinant protein D. 0.5 ug His-recombinant protein E. 0.1 ug His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT161] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
|
|