6X His tag Antibody (OAGA08352)

Data Sheet
 
Product Number OAGA08352
Product Page www.avivasysbio.com/6x-his-tag-antibody-oaga08352.html
Name 6X His tag Antibody (OAGA08352)
Clone GT161
Isotype IgG2a
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAGA08352
Application Info Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer PBS
Purification Affinity purified by Protein G.
Gene Symbol 6X His tag
Application WB
Image 1
His-tagged recombinant proteins
6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/ml 6X His tag antibody [GT161] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.
Image 2
E. coli
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12% SDS-PAGE
6X His tag antibody [GT161] dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Image 3
E. coli
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. Non-induced cell lysate from E. coli expressing His-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins
12% SDS-PAGE
6X His tag antibody [GT161] dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Image 4
GST-recombinant protein
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. 2 ug GST-recombinant protein
B. 2 ug His-recombinant protein
C. 1 ug His-recombinant protein
D. 0.5 ug His-recombinant protein
E. 0.1 ug His-recombinant protein
12% SDS-PAGE
6X His tag antibody [GT161] dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
 

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