| Product Number |
OAGA07499 |
| Product Page |
www.avivasysbio.com/arid1a-antibody-oaga07499.html |
| Name |
ARID1A Antibody (OAGA07499) |
| Isotype |
IgG |
| NCBI Gene Id |
8289 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.09 mg/ml |
| Gene Full Name |
AT-rich interaction domain 1A |
| Alias Symbols |
ELD, B120, CSS2, OSA1, P270, hELD, BM029, MRD14, hOSA1, BAF250, C1orf4, BAF250a, SMARCF1 |
| Product Format |
Liquid |
| Description of Target |
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAGA07499 |
| Application Info |
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary. |
| Storage Buffer |
0.2M Tris, 0.2M Glycine, 1mM EGTA, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Uniprot ID |
O14497 |
| Protein Name |
AT-rich interactive domain-containing protein 1A |
| Protein Accession # |
NP_006006.3 |
| Purification |
Purified by antigen-affinity chromatography. |
| Nucleotide Accession # |
NM_006015.4 |
| Preservative |
0.025% ProClin 300 |
| Gene Symbol |
ARID1A |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, IHC-P, IP, WB |
| Image 1 | HeLa cells
 | ARID1A antibody detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID1A protein stained by ARID1A antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
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| Image 2 | Human cervical carcinoma
 | ARID1A antibody detects ARID1A protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ARID1A antibody dilution: 1:500.
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| Image 3 | Human endometrial carcinoma
 | ARID1A antibody detects ARID1A protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
ARID1A antibody diluted at 1:500.
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| Image 4 | HepG2 whole cell
 | ARID1A antibody immunoprecipitates ARID1A protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of ARID1A protein by 4 ug ARID1A antibody
5 % SDS-PAGE
The immunoprecipitated ARID1A protein was detected by ARID1A antibody diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
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| Image 5 | Whole cell
 | ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody diluted by 1:1000. |
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| Image 6 | Whole cell
 | ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody diluted by 1:1000. |
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| Image 7 | Various whole cell
 | ARID1A antibody detects ARID1A protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody diluted by 1:1000. |
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| Image 8 | HepG2 whole cell
 | ARID1A antibody detects ARID1A protein by western blot analysis.
A. 30 ug HepG2 whole cell extract
B. 30 ug HepG2 nuclear extract
5 % SDS-PAGE
ARID1A antibody dilution: 1:1000 |
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