mCherry Antibody (OAGA07337)

Data Sheet
 
Product Number OAGA07337
Product Page www.avivasysbio.com/mcherry-antibody-oaga07337.html
Name mCherry Antibody (OAGA07337)
Isotype IgG
Host Rabbit
Clonality Polyclonal
Concentration 0.36 mg/ml
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAGA07337
Application Info Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-Fr, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Preservative 0.025% ProClin 300
Gene Symbol mCherry
Application ICC, IF, IHC-F, IP, WB, IHC
Image 1
HeLa cells
mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis.
Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min.
Green: mCherry protein stained by mCherry antibody diluted at 1:500.
Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
Image 2
Zebrafish embryo
mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells dilution: 1:100.
Image 3
293T whole cell
mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Image 4
MCherry
mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Image 5
293T whole cell
mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
 

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