Product Number |
OAGA07337 |
Product Page |
www.avivasysbio.com/mcherry-antibody-oaga07337.html |
Name |
mCherry Antibody (OAGA07337) |
Isotype |
IgG |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.36 mg/ml |
Product Format |
Liquid |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAGA07337 |
Application Info |
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-Fr, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Storage Buffer |
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Purification |
Purified by antigen-affinity chromatography. |
Preservative |
0.025% ProClin 300 |
Gene Symbol |
mCherry |
Application |
ICC, IF, IHC-F, IP, WB, IHC |
Image 1 | HeLa cells
| mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis. Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min. Green: mCherry protein stained by mCherry antibody diluted at 1:500. Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
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| Image 2 | Zebrafish embryo
| mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. mCherry antibody labels mCherry-expressing cells dilution: 1:100.
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| Image 3 | 293T whole cell
| mcherry antibody immunoprecipitates mcherry protein in IP experiments.
IP Sample: 293T whole cell lysate/extract
A : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cells
B : Control with 2.5 ug of pre-immune rabbit IgG
C : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody
7.5% SDS-PAGE
The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000.
EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent. |
| Image 4 | MCherry
| mCherry antibody detects mCherry (full length) protein by western blot analysis. A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12% SDS-PAGE mCherry antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. |
| Image 5 | 293T whole cell
| mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. |
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