VPS35 Antibody (OAGA05354)

• Product Number: OAGA05354
• Name: VPS35 Antibody (OAGA05354)
• Isotype: IgG
• NCBI Gene Id: 55737
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.64 mg/ml
• Gene Full Name: VPS35, retromer complex component
• Alias Symbols: MEM3, PARK17
• Product Format: Liquid
• Description of Target: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Application Info: Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Uniprot ID: Q96QK1
• Protein Name: vacuolar protein sorting-associated protein 35
• Protein Accession #: NP_060676.2
• Purification: Purified by antigen-affinity chromatography.
• Nucleotide Accession #: NM_018206.5
• Preservative: 0.01% Thimerosal
• Gene Symbol: VPS35
• Predicted Species Reactivity: Human, Mouse
• Application: IHC-P, IP, WB
• Image 1: A549 xenograft
  
  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS35 antibody at 1:500 dilution.
• Image 2: HeLa whole cell
  
  Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody .
  Western blot analysis was performed using VPS35 antibody .
  EasyBlot anti-Rabbit IgG was used as a secondary reagent.
• Image 3: NIH-3T3
  
  Sample (30 ug of whole cell lysate)
  A: NIH-3T3
  B: JC
  7.5% SDS PAGE
  diluted at 1:1000
  The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
• Image 4: HCT116
  
  Sample (30 ug of whole cell lysate)
  A: HCT116
  7.5% SDS PAGE
  diluted at 1:1000
  The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.