| Product Number |
OAGA04026 |
| Product Page |
www.avivasysbio.com/acly-antibody-n-terminal-region-oaga04026.html |
| Name |
ACLY Antibody - N-terminal region (OAGA04026) |
| Molecular Weight |
121 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
47 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Varies by lot. See vial for concentration. |
| Gene Full Name |
ATP citrate lyase |
| Description |
|
| Alias Symbols |
ACL, ATPCL, CLATP |
| Product Format |
Liquid 1X PBS, 20% glycerol (pH 7) with 0.025% Proclin300 added as a preservative |
| Description of Target |
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. |
| Reconstitution and Storage |
Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAGA04026 |
| Application Info |
WB: 1:1000~10000
ICC/IF: 1:100~1000
IHC-P: 1:100~1000 |
| Immunogen |
Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary. |
| Uniprot ID |
P53396 |
| Protein Name |
ATP-citrate synthase |
| Publications |
ATP-citrate lyase promotes axonal transport across species. Nat Commun. 12, 5878 (2021) |
| Protein Accession # |
NP_001087.2 |
| Purification |
Purified by antigen-affinity chromatography. |
| Nucleotide Accession # |
NM_001096.2 |
| Gene Symbol |
ACLY |
| Predicted Species Reactivity |
Human, Mouse, Rat |
| Application |
WB, ICC, IF, IHC-P |
| Image 1 | HeLa cells
 | ATP citrate lyase antibody [N1N2], N-term detects ACLY protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACLY protein stained by ATP citrate lyase antibody [N1N2], N-term diluted at 1:500.
Blue: Hoechst 33343 staining.
|
| | Image 2 | Hepatoma
 | Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase antibody at 1:500 dilution. |
| | Image 3 | 293T whole cell
 | Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: Jurkat
D: Raji
5% SDS PAGE
diluted at 1:5000
|
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