| Product Number |
OAGA03475 |
| Product Page |
www.avivasysbio.com/eif2b5-antibody-oaga03475.html |
| Name |
EIF2B5 Antibody (OAGA03475) |
| Isotype |
IgG |
| NCBI Gene Id |
8893 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
eukaryotic translation initiation factor 2B subunit epsilon |
| Alias Symbols |
CLE, CACH, LVWM, EIF-2B, EIF2Bepsilon |
| Product Format |
Liquid |
| Description of Target |
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAGA03475 |
| Application Info |
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human EIF2B epsilon. The exact sequence is proprietary. |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q13144 |
| Protein Name |
translation initiation factor eIF-2B subunit epsilon |
| Protein Accession # |
NP_003898.2 |
| Purification |
Purified by antigen-affinity chromatography. |
| Nucleotide Accession # |
NM_003907.2 |
| Preservative |
0.01% Thimerosal |
| Gene Symbol |
EIF2B5 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
ICC, IF, WB |
| Image 1 | Paraformaldehyde-fixed A431
 | Immunofluorescence analysis of paraformaldehyde-fixed A431, using eIF2B epsilon antibody at 1:200 dilution. |
| | Image 2 | Mouse liver
 | Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
diluted at 1:1000
|
| | Image 3 | H1299
 | Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
Diluted at 1:1000 |
|
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