| Product Number |
OAGA03463 |
| Product Page |
www.avivasysbio.com/mcm4-antibody-oaga03463.html |
| Name |
MCM4 Antibody (OAGA03463) |
| Isotype |
IgG |
| NCBI Gene Id |
4173 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
minichromosome maintenance complex component 4 |
| Alias Symbols |
NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21 |
| Product Format |
Liquid |
| Description of Target |
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAGA03463 |
| Application Info |
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:10000, ChIP assay. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary. |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
P33991 |
| Protein Name |
DNA replication licensing factor MCM4 |
| Protein Accession # |
NP_005905.2 |
| Purification |
Purified by antigen-affinity chromatography. |
| Nucleotide Accession # |
NM_005914.3 |
| Preservative |
0.01% Thimerosal |
| Gene Symbol |
MCM4 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
ICC, IF, IHC-P, IP, WB, CHIP |
| Image 1 | HeLa
 | Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter. |
|
| Image 2 | Paraformaldehyde-fixed HeLa
 | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM4 antibody at 1:500 dilution. |
|
| Image 3 | A549 Xenograft
 | Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4 antibody at 1:100 dilution. |
|
| Image 4 | Jurkat whole cell
 | MCM4 antibody immunoprecipitates MCM4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 ug Jurkat whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of MCM4 protein by 4 ug MCM4 antibody
7.5 % SDS-PAGE
The immunoprecipitated MCM4 protein was detected by MCM4 antibody diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
|
|
| Image 5 | Various whole cell
 | Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000. |
|
| Image 6 | Jurkat whole cell
 | MCM4 antibody detects MCM4 protein by western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug Raji whole cell lysate/extract
C. 30 ug NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
MCM4 antibody dilution: 1:5000 |
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