| Product Number |
OAGA02151 |
| Product Page |
www.avivasysbio.com/znf45-antibody-oaga02151.html |
| Name |
ZNF45 Antibody (OAGA02151) |
| Isotype |
IgG |
| NCBI Gene Id |
7596 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5mg/ml |
| Gene Full Name |
zinc finger protein 45 |
| Alias Symbols |
KOX5, ZNF13 |
| Product Format |
Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-ZNF45 (OAGA02151) antibody |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human ZNF45. The exact sequence is proprietary. |
| Uniprot ID |
Q02386 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
ZNF45 |
| Predicted Species Reactivity |
Human |
| Application |
IF, IHC-P, WB |
| Image 1 | A375
 | ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF45 protein stained by ZNF45 antibody [N2C3] (OAGA02151) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
| | Image 2 | HCT116
 | ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF45 protein stained by ZNF45 antibody [N2C3] (OAGA02151) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
| | Image 3 | Human colon carcinoma
 | ZNF45 antibody [N2C3] detects ZNF45 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ZNF45 antibody [N2C3] (OAGA02151) dilution: 1:250.
|
| | Image 4 | 293T
 | Sample (30 ug of whole cell lysate)
A:293T
7.5% SDS PAGE
OAGA02151 diluted at 1:2000 |
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