| Product Number |
OAGA02126 |
| Product Page |
www.avivasysbio.com/nfe2l2-antibody-middle-region-oaga02126.html |
| Name |
NFE2L2 Antibody - middle region (OAGA02126) |
| Molecular Weight |
68 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
4780 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.11 mg/ml |
| Gene Full Name |
nuclear factor, erythroid 2-like 2 |
| Alias Symbols |
NRF2, HEBP1, Nrf-2, IMDDHH |
| Product Format |
Liquid. 1XPBS, 1% BSA, 20% Glycerol (pH 7). 0.025% ProClin 300 was added as a preservative. |
| Description of Target |
NFE2 , NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, although remaining regions have diverged considerably from each other. |
| Reconstitution and Storage |
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below.
Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NFE2L2 (OAGA02126) antibody |
| Additional Information |
Positive Controls: C8D30 , 3xFlag-human NFE2L2-transfected 293T , MDA-MB-231 nuclear extract , MDA-MB-231 nuclear extract (30uM tBHQ, 4hr) , HepG2 (10 uM MG132 treatment for 15 hr)
Cellular Localization: Cytoplasm , cytosol , Nucleus |
| Application Info |
ICC/IF: 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB 1:500-1:3000
CHIP: Assay-dependent dilution
IHC: Assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the researcher. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary. |
| Uniprot ID |
Q16236 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
NFE2L2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Alligator, Bird, Plant |
| Application |
ICC, IF, IHC, IHC-FFPE, IP, WB, ChIP assay |
| Predicted Homology Based on Immunogen Sequence |
Rhesus Monkey (99%), Bovine (84%) |
| Image 1 | Paraffin-embedded Cal27 xenograft
 | Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2 (OAGA02126) antibody at 1:500 dilution. |
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| Image 2 | 293T
 | NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (OAGA02126) diluted by 1:1000. |
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