| Product Number |
OAGA01617 |
| Product Page |
www.avivasysbio.com/pou5f1-antibody-oaga01617.html |
| Name |
POU5F1 Antibody (OAGA01617) |
| Isotype |
IgG |
| NCBI Gene Id |
5460 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1mg/ml |
| Gene Full Name |
POU class 5 homeobox 1 |
| Alias Symbols |
OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4 |
| Product Format |
Liquid. 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Description of Target |
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-POU5F1 (OAGA01617) antibody |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:5000-1:50000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:500-1:1000, FACS: 1:50-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary. |
| Uniprot ID |
Q01860 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
POU5F1 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
FACS, IF, IHC-P, IP, WB |
| Image 1 | CF1 feeders
 | Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4 (OAGA01617) antibody at 1:200 dilution. |
|
| Image 2 | CF1 feeders
 | Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4 (OAGA01617) antibody at 1:200 dilution. |
|
| Image 3 | Paraffin-embedded Cal27 xenograft
 | Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OCT3/4 (OAGA01617) antibody at 1:500 dilution. |
|
| Image 4 | Transfected 293T cells
 | Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 ug of Oct4 antibody (OAGA01617) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (OAGA01617) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent. |
|
| Image 5 | Various whole cell extracts
 | OCT4 antibody detects OCT4 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT4 antibody (OAGA01617) at a dilution of 1:50000. |
|
| Image 6 | Human ESC
 | Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 ug human ESC whole cell extract
12 % SDS-PAGE
Oct4 antibody (OAGA01617) dilution: 1:10000 |
|
| Image 7 | Mouse ESC
 | Sample (20 ug of whole cell lysate)
A: mouse ESC
12% SDS PAGE
OAGA01617 diluted at 1:10000
|
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