LYN Antibody (OAGA01520)

Data Sheet
 
Product Number OAGA01520
Product Page www.avivasysbio.com/lyn-antibody-oaga01520.html
Name LYN Antibody (OAGA01520)
Isotype IgG
NCBI Gene Id 4067
Host Rabbit
Clonality Polyclonal
Concentration 1mg/ml
Gene Full Name LYN proto-oncogene, Src family tyrosine kinase
Alias Symbols JTK8, p53Lyn, p56Lyn
Product Format Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Description of Target This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-LYN (OAGA01520) antibody
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.
Uniprot ID P07948
Purification Purified by antigen-affinity chromatography.
Gene Symbol LYN
Predicted Species Reactivity Human
Application IF, IHC-P, IP, WB
Image 1
Paraformaldehyde-fixed A431
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN (OAGA01520) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.
Image 2
Paraffin-embedded Colon ca
Immunohistochemical analysis of paraffin-embedded Colon ca, using LYN (OAGA01520) antibody at 1:250 dilution.
Image 3
K562
LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of LYN protein by 4 ug LYN antibody (OAGA01520)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (OAGA01520) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Image 4
Raji, K562
LYN antibody detects LYN protein by Western blot analysis.
A. 30 ug Raji whole cell lysate/extract
B. 30 ug K562 whole cell lysate/extract
C. 30 ug Raji whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (OAGA01520) dilution: 1:1000
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com