Product Number |
OAGA01520 |
Product Page |
www.avivasysbio.com/lyn-antibody-oaga01520.html |
Name |
LYN Antibody (OAGA01520) |
Isotype |
IgG |
NCBI Gene Id |
4067 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1mg/ml |
Gene Full Name |
LYN proto-oncogene, Src family tyrosine kinase |
Alias Symbols |
JTK8, p53Lyn, p56Lyn |
Product Format |
Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Description of Target |
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-LYN (OAGA01520) antibody |
Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Immunogen |
Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary. |
Uniprot ID |
P07948 |
Purification |
Purified by antigen-affinity chromatography. |
Gene Symbol |
LYN |
Predicted Species Reactivity |
Human |
Application |
IF, IHC-P, IP, WB |
Image 1 | Paraformaldehyde-fixed A431
| Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN (OAGA01520) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000. |
| Image 2 | Paraffin-embedded Colon ca
| Immunohistochemical analysis of paraffin-embedded Colon ca, using LYN (OAGA01520) antibody at 1:250 dilution. |
| Image 3 | K562
| LYN antibody immunoprecipitates LYN protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of LYN protein by 4 ug LYN antibody (OAGA01520) 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody (OAGA01520) diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
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| Image 4 | Raji, K562
| LYN antibody detects LYN protein by Western blot analysis. A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract C. 30 ug Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody (OAGA01520) dilution: 1:1000 |
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