| Product Number |
OAGA01482 |
| Product Page |
www.avivasysbio.com/lmna-antibody-oaga01482.html |
| Name |
LMNA Antibody (OAGA01482) |
| Isotype |
IgG |
| NCBI Gene Id |
4000 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.23mg/ml |
| Gene Full Name |
lamin A/C |
| Alias Symbols |
FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, MADA, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B |
| Product Format |
Liquid. 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Description of Target |
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-LMNA (OAGA01482) antibody |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary. |
| Uniprot ID |
P02545 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
LMNA |
| Predicted Species Reactivity |
Human, Mouse, Rat |
| Application |
IF, IHC-P, IP, WB |
| Image 1 | Methanol-fixed Hela
 | Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C (OAGA01482) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:500. |
|
| Image 2 | Paraffin-embedded C2C12 xenograft
 | Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C (OAGA01482) antibody at 1:500 dilution. |
|
| Image 3 | Paraffin-embedded Mahlarvu xenograft
 | Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C (OAGA01482) antibody at 1:500 dilution. |
|
| Image 4 | Paraffin-embedded RT2 xenograft
 | Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C (OAGA01482) antibody at 1:500 dilution. |
|
| Image 5 | Hela
 | Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 ug HeLa whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (OAGA01482)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (OAGA01482) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
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| Image 6 | Various whole cell extracts
 | Lamin A + C antibody detects Lamin A + C protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (OAGA01482) diluted by 1:2000. |
|
| Image 7 | NIH-3T3
 | Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
OAGA01482 diluted at 1:1000
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