| Product Number |
OAGA01293 |
| Product Page |
www.avivasysbio.com/muc1-antibody-oaga01293.html |
| Name |
MUC1 Antibody (OAGA01293) |
| Isotype |
IgG |
| NCBI Gene Id |
4582 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1mg/ml |
| Gene Full Name |
mucin 1, cell surface associated |
| Alias Symbols |
EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADTKD2, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC |
| Product Format |
Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Description of Target |
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MUC1 (OAGA01293) antibody |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human MUC1. The exact sequence is proprietary. |
| Uniprot ID |
P15941 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
MUC1 |
| Predicted Species Reactivity |
Human |
| Application |
IF, IHC-P, IP, WB |
| Image 1 | Methanol-fixed A431
 | Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUC1 (OAGA01293) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:500. |
| | Image 2 | Paraffin-embedded human cervical cancer
 | MUC1 antibody detects MUC1 protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
MUC1 antibody (OAGA01293) diluted at 1:500.
|
| | Image 3 | HCT116
 | Immunoprecipitation of MUC1 protein from HCT-116 whole cell extracts using 5 ug of MUC1 antibody (OAGA01293).
Western blot analysis was performed using MUC1 antibody (OAGA01293).
EasyBlot anti-Rabbit IgG was used as a secondary reagent. |
| | Image 4 | HCT116
 | Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
OAGA01293 diluted at 1:5000
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