| Product Number |
OAGA01132 |
| Product Page |
www.avivasysbio.com/edc3-antibody-oaga01132.html |
| Name |
EDC3 Antibody (OAGA01132) |
| Molecular Weight |
56 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
80153 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Enhancer of mRNA-decapping protein 3 |
| Alias Symbols |
YJDC, LSM16, MRT50, YJEFN2, hYjeF_N2-15q23 |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-EDC3 antibody - OAGA01132 |
| Additional Information |
Background: EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM] |
| Additional Information |
Positive Controls: A431, NIH-3T3, C2C12 |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 127 and 376 of Edc3 (Uniprot ID#Q96F86) |
| Additional Information |
Cellular Localization: Cytoplasm, P-body |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q96F86 |
| Protein Name |
Enhancer of mRNA-decapping protein 3 |
| Protein Accession # |
NP_001135915.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
EDC3 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
IHC-FFPE, WB |
| Image 1 | A431
 | Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
OAGA01132 diluted at 1:3000
|
| | Image 2 | NIH-3T3
 | Edc3 antibody [N2C2], Internal detects EDC3 protein by Western blot analysis.
A. 30 ug NIH-3T3 whole cell lysate/extract
B. 30 ug C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Edc3 antibody [N2C2], Internal (OAGA01132) dilution: 1:1000 |
| | Image 3 | MCF7 Xenograft
 | Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Edc3(OAGA01132) antibody at 1:250 dilution. |
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