| Product Number |
OAGA01058 |
| Product Page |
www.avivasysbio.com/pmm2-antibody-oaga01058.html |
| Name |
PMM2 Antibody (OAGA01058) |
| Molecular Weight |
28 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
5373 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.24 mg/ml |
| Gene Full Name |
Phosphomannomutase 2 |
| Alias Symbols |
PMI, CDG1, CDGS, PMI1, CDG1a, PMM 2 |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PMM2 antibody - OAGA01058 |
| Additional Information |
Background: The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq] |
| Additional Information |
Positive Controls: 293T, HeLa, HepG2 |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 214 of PMM2 (Uniprot ID#O15305) |
| Additional Information |
Cellular Localization: Cytoplasm |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
O15305 |
| Protein Name |
Phosphomannomutase 2 |
| Protein Accession # |
NP_000294.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
PMM2 |
| Predicted Species Reactivity |
Human |
| Application |
IHC-FFPE, WB |
| Image 1 | Hela , HepG2
 | Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
12% SDS PAGE
OAGA01058 diluted at 1:1000
|
| | Image 2 | U87 Xenograft
 | Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PMM2(OAGA01058) antibody at 1:100 dilution. |
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