Product Number |
OAGA00965 |
Product Page |
www.avivasysbio.com/dtx1-antibody-oaga00965.html |
Name |
DTX1 Antibody (OAGA00965) |
Molecular Weight |
67 kDa |
Isotype |
IgG |
NCBI Gene Id |
1840 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
E3 ubiquitin-protein ligase DTX1 |
Description |
|
Alias Symbols |
hDx-1, RNF140 |
Product Format |
Liquid. 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7). 0.01% Thimerosal was added as a preservative. |
Description of Target |
Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. |
Reconstitution and Storage |
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-DTX1 antibody - OAGA00965 |
Application Info |
WB: 1:500-1:3000
ICC: 1:100-1:1000
IF: 1:100-1:1000
IHC-P: 1:100-1:1000
Optimal dilutions should be determined by the end user. |
Immunogen |
Recombinant protein encompassing a sequence within the center region of human Deltex1. The exact sequence is proprietary. |
Uniprot ID |
Q86Y01 |
Protein Name |
E3 ubiquitin-protein ligase DTX1 |
Publications |
Glycogen synthase kinase 3β inhibition enhances Notch1 recycling. Mol Biol Cell. 29, 389-395 (2018). 29237816
PI5P4Kγ functions in DTX1-mediated Notch signaling. Proc Natl Acad Sci U S A. 115, E1983-E1990 (2018). 29440432 |
Protein Accession # |
NP_004407.2 |
Purification |
Purified by antigen-affinity chromatography. |
Tested Species Reactivity |
Human |
Gene Symbol |
DTX1 |
Predicted Species Reactivity |
Mouse, Cow, Xenopus laevis |
Application |
WB, ICC, IF, IHC-P |
Image 1 | Hela
| Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE OAGA00965 diluted at 1:1000
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| Image 2 | BT474 Xenograft
| Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Deltex1(OAGA00965) antibody at 1:500 dilution. |
| Image 3 | HeLa
| Immunofluorescence analysis of methanol-fixed HeLa, using Deltex1(OAGA00965) antibody at 1:200 dilution. |
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